Left: Human blood plasma containing different types of cells.
Middle: Polymorphonuclear human white blood cells. After separation, cells were plated on the QX-102 capsule membrane. Efficient attachment and spreading of the population of interest were achieved. Fixation and consequent Uranyl Acetate and Osmium Tetroxide staining enhanced visualization of cellular organelles, and highlighted the lipid bodies inside the cytoplasm. These lipid bodies are known to be accumulated in circumstances such as inflammation.
Right: Red blood cells (Erythrocytes) were plated on a poly-l-lysine coated QX-102 capsule membrane using a centrifuge at 3000 rpm for five minutes. Cells were then fixed with 0.2% Glutaraldehyde and stained with 0.5% Osmium Tetroxide for 30 min.