Microorganisms / General staining
1Wet T. brucei stained with osmium tetroxide
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Fully hydrated Trypanosoma brucei were attached to the QX-capsule membrane and stained with 1% osmium tetroxide. Lipid-rich structures are heavily stained and are visible as white vesicles.

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2Wet B. subtilis stained with uranyl acetate
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Fully hydrated B. subtilis bacteria were attached to the QX-capsule membrane, fixed with 4% paraformaldehyde/PBS for 10 min and stained with 0.1% uranyl acetate.

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3Trypanosoma brucei stained with uranyl acetate
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Fixed Trypanosoma brucei stained with uranyl acetate was attached to the membrane of a QX-102 capsule. Sample was imaged using LEO 1550 VP.

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4Bacteria suspension
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A bacteria suspension was incubated on a poly-l-lysine coated QX-102 capsule and stained with uranyl acetate

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5Epithelial cells with Staphylococus bacteria
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MACT cells (epithelial cells from mammary gland) were cultured on a Fibronectin coated QX-102 membrane, and then infected with Staphylococcus aureus. In these images we can see the initial attachment of staphilococus aerus to external extensions of MACT cells (left), and how the bacteria is eventually incorporated into the cells (right). By growing the cells and bacteria directly in the QX-102 capsule artifacts are minimized and the system can be imaged in its native environment. (Courtesy of Dr. Kasia Dziewanowska University of Idaho.)

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6Bacterial biofilm on latex surface
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117
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Aggregation of bacteria on latex tubing, forming a biofilm. The sample was stained with Uranyl Acetate and imaged in its fully hydrate state by using the QX-302 capsule, eliminating the risk of dehydration artifacts.

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