Tissues / General staining
1Wet rabbit retinal pigment epithelium
73
 click to enlarge
73
 click to enlarge

Retinal pigment epithelium from a rabbit eye fixed in formalin and stained with 0.1% osmium tetroxide for 30 min. The melanosomes, the pigment-containing organelles within the epithelial villi, are seen as bright cigar-shaped structures. Left: Overall structure of the epithelial cells, covered with the melanosomes, is seen at lower magnification. The cell nuclei are visible as the brighter spheres. Bar: 10um.
Right: A closer look at the melanosomes. Bar: 2um.

[go back]

 
2Wet corneal endothelial Cells
84
 click to enlarge
84
 click to enlarge

Wet corneal endothelium from a rabbit eye fixed in formalin and stained for 10 minutes with 0.1% uranyl acetate.
Left: Overall structure of the convoluted endothelial cells is seen at lower magnification. Bar: 50um.
Right: higher magnification. Bar: 10um.

[go back]

 
3Wet mucosal surface of intestines
86
 click to enlarge
86
 click to enlarge
86
 click to enlarge

Human epithelium fixed in formalin and stained with 0.1% uranyl acetate for 10 min.
Bars: left 100um, middle 50 um, right 20 um.

[go back]

 
4Wet skeletal muscle
90
 click to enlarge

Porcine skeletal muscle fixed in formalin and stained for 10 min with 0.1% uranyl acetate. The striations are clearly visible, including internal structures, such as the Z-bands. Bar: 2um.

[go back]

 
5Wet rabbit lens
92
 click to enlarge

Lens from a rabbit eye fixed in formalin and stained with 0.1% osmium tetroxide for 10 min. Bar: 5um

[go back]

 
6Rat sciatic nerve stained with osmium tetroxide
94
 click to enlarge
94
 click to enlarge

Rat sciatic nerve was fixed with formalin and stained with 0.1% osmium tetroxide for 30 min. Cross section was imaged using the QX-302 capsule.

[go back]

 
7Comparison between normal bowel and IBD (Inflammatory Bowel Disease)
100
 click to enlarge
100
 click to enlarge

Tissue samples were stained with uranyl acetate and imaged fully hydrated with QX-302 capsule. Left: Normal Bowel, Right: IBD (Crohn disease).The normal units of the epithelial layer lose their regular polygonal cell borders in the IBD.
(Iris Barshak et al. "A Novel Method for 'Wet' SEM." Ultrastructural Pathology, Volume 28, Number 1 / January-February 04.)

[go back]

 
8Progression of lipid accumulation in tissues
118
 click to enlarge
118
 click to enlarge

Lipid body accumulation (right) in longitudinal sections of skeletal muscles. Samples were obtained from diet-induced diabetes in sand rats (Psammomys Obesus) after three weeks on high-energy diet. Increased quantity and size of lipids is observed when compared with the control animal (left). The skeletal tissue is one of the first tissues to accumulate lipid bodies with the onset of insulin resistance. (In collaboration with Prof. I. Raz, Hadassa Medical Center, Israel).

[go back]

 
9Myelin disorders multiple sclerosis model
119
 click to enlarge
119
 click to enlarge

Morphometric quantification of Myelin loss.
A comparison of cervical spinal cord sections from a control animal (left) and from an SCH-immunized Biozzi mouse (right) that developed severe grade of clinical disease, a model for the human multiple sclerosis (MS) disease.
The myelin-containing white matter appears as dark rings. Hardly any intact myelin rings were noted in the affected areas and instead the tissue is infiltrated by clearly nucleated cells (nuclei appear bright). (In collaboration with TEVA Pharmacuticals)

[go back]

 
10Liver tissue
120
 click to enlarge
120
 click to enlarge

Hepatocytes of a rat .The sample was stained with 1% phosphotungstic acid.

[go back]

 
11Glomerulus in rat kidney
121
 click to enlarge
121
 click to enlarge

A kidney section from a rat showing normal aspects of the glomerulus with podocytes, urinary space, mesangial matrix and basement membrane. The sample was stained with uranyl acetate.

[go back]